• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文OA文献 >BMP-5 expression increases during chondrocyte differentiation in vivo and in vitro and promotes proliferation and cartilage matrix synthesis in primary chondrocyte cultures
【2h】

BMP-5 expression increases during chondrocyte differentiation in vivo and in vitro and promotes proliferation and cartilage matrix synthesis in primary chondrocyte cultures

机译：Bmp-5表达在体内和体外软骨细胞分化期间增加，并促进原代软骨细胞培养物中的增殖和软骨基质合成。

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Bone morphogenetic proteins (BMPs) play pivotal roles in bone and cartilage growth and repair. Through phenotypes of short-ear (se) mice, which have BMP-5 mutations, a role for BMP-5 in some specific aspects of skeletogenesis and cartilage growth is known. This report examines BMP-5 expression in the growth plate and in differentiating cultures of primary chondrocytes, and the effects of addition of BMP-5 or its inhibition by anti-BMP-5 antibody in chondrocyte cultures. By laser capture microdissection and immunohistochemistry, we found that BMP-5 is expressed in proliferating zone (PZ) chondrocytes and that the expression increases sharply with hypertrophic differentiation. A similar pattern was observed in differentiating cultures of primary chondrocytes, with BMP-5 expression increasing as cells differentiated, in contrast to other BMPs. BMP-5 added to cultures increased cell proliferation early in the culture period and also stimulated cartilage matrix synthesis. Also, BMP-5 addition to the cultures activated phosphorylation of Smad 1/5/8 and p38 MAP kinase and caused increased nuclear accumulation of phospho-Smads. Anti-BMP-5 antibody inhibited the endogenous BMP-5, reducing cell proliferation and phospho-Smad nuclear accumulation. Together, the results demonstrate that BMP-5 is normally an important regulator of chondrocyte proliferation and differentiation. Whether other BMPs may compensate in BMP-5 loss-of-function mutations is discussed.
机译：骨形态发生蛋白（BMP）在骨骼和软骨的生长和修复中起关键作用。通过具有BMP-5突变的短耳（se）小鼠的表型，已知BMP-5在骨骼生成和软骨生长的某些特定方面的作用。该报告研究了软骨细胞在生长板和分化培养物中的BMP-5表达，以及软骨细胞培养物中BMP-5的添加或抗BMP-5抗体的抑制作用。通过激光捕获显微切割和免疫组织化学，我们发现BMP-5在增生区（PZ）软骨细胞中表达，并且该表达随着肥大性分化而急剧增加。在分化原代软骨细胞的培养物中观察到相似的模式，与其他BMP相比，随着细胞分化，BMP-5表达增加。向培养物中添加BMP-5可以在培养早期增加细胞增殖，还可以刺激软骨基质的合成。同样，向培养物中添加BMP-5可以激活Smad 1/5/8和p38 MAP激酶的磷酸化，并导致磷酸Smads的核积累增加。抗BMP-5抗体抑制内源性BMP-5，减少细胞增殖和磷酸Smad核积累。总之，结果表明BMP-5通常是软骨细胞增殖和分化的重要调节剂。讨论了其他BMP是否可以补偿BMP-5功能丧失突变。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号